The best thing to do is to use relative paths for all your assets. This way it is easy to move the project to another machine. Everything works the same for TouchPlayer.
The folder where the .toe file resides is called the project folder.
Place all your assets in subdirectories of this folder, for instance a folder “assets” which has another subdirectory “movies”.
Then load your assets in the Movie File in TOP(and others) by using a file path like: assets/movies/mymovie.mov
Also you can get the path of the current project folder in Python by using project.folder
See the Project Class wiki page